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KMID : 1098420090170040293
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Korean Journal of Medicinal Crop Science
2009 Volume.17 No. 4 p.293 ~ p.300
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Effect of the BuOH Soluble Fraction of Cinnamomum camphora on Melanin Biosynthesis
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Ha Sang-Keun
Kim Sun-Yeou
Yoo Eun-Sook
Oh Myung-Sook
Moon Eun-Jung
Lee Min-Jae
Park Hye-Min
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Abstract
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This study was carried out to investigate the effect of Cinnamomum camphora on melanogenesis. The MeOH extract of Cinnamomum camphora inhibited mushroom tyrosinase activity in dose-dependent manner. Moreover, it significantly suppressed the melanin production in melan-a cells at the concentration of . The MeOH extract was partitioned with ethyl acetate, n-butanol and water. Among them, the BuOH soluble fraction exhibited significant inhibitory effect on mushroom tyrosinase. In addition, the BuOH soluble fraction reduced the melanin production in melan-a cells. But, the BuOH soluble fraction had less inhibition effects on melan-a cell originated tyrosinase. So, it was performed western blotting for melanogenic proteins (tyrosinase, tyrosinase-related protein (TRP-2)) using melan-a cells. The BuOH soluble fraction inhibited the protein expression of tyrosinase at the concentration of . The results suggested that the BuOH soluble fraction of C. camphora might be a potent inhibitor of melanin biosynthesis in melan-a cells.
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KEYWORD
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Melanin, Tyrosinase, Cinnamomum camphora
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